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Literature summary extracted from

  • Wang, P.; Doxtader, K.A.; Nam, Y.
    Structural basis for cooperative function of Mettl3 and Mettl14 methyltransferases (2016), Mol. Cell, 63, 306-317 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.348 expression in Escherichia coli Homo sapiens

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.1.1.348 crystal structures of the complex of Mettl3/Mettl14 methyltransferase domains in apo form as well as with bound S-adenosylmethionine (SAM) or S-adenosylhomocysteine (SAH) in the catalytic site. The heterodimeric complex of methyltransferase domains, combined with CCCH motifs constitute the minimally required regions for creating m6A modifications in vitro. Mettl3 is the catalytically active subunit while Mettl14 plays a structural role critical for substrate recognition Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.348 D395A change of the DPPW catalytic motif to APPW in METTL3, abrogates the ability of its complex with wild type METTL14 to methylate RNA Homo sapiens
2.1.1.348 E192A change of EPPL to APPL in METTL14, little effect on methylation capacity if wild type METTL3 is present Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.348 S-adenosyl-L-methionine + adenine in mRNA Homo sapiens
-
 S-adenosyl-L-homocysteine + N6-methyladenine in mRNA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.348 Homo sapiens Q86U44 and Q9HCE5 Q86U44 i.e. catalytic subunit METTL3, Q9HCE5 i.e non-catalytic subunit METTL14
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.348 additional information consensus sequence for methylation of RNA is GGACU. The single transition mutation, GGACU to GGAUU, is sufficient to significantly reduce methylation levels Homo sapiens ?
-
 ?
2.1.1.348 S-adenosyl-L-methionine + adenine in 5'-UACACUCGAUCU-GGACU-AAAGCUGCUC
-
 Homo sapiens homocysteine + 5'-UACACUCGAUCU-GG-(N6-methyl)ACU-AAAGCUGCUC
-
 ?
2.1.1.348 S-adenosyl-L-methionine + adenine in mRNA
-
 Homo sapiens S-adenosyl-L-homocysteine + N6-methyladenine in mRNA
-
 ?